Research

Ruthenium-Based Therapeutics

Combining bench-top synthesis with quantum chemistry, molecular docking, and machine learning to design the next generation of metal-based anticancer and antimycobacterial agents.

A Two-Pronged Research Strategy

My PhD research centres on ruthenium(III) and half-sandwich Ru–arene complexes bearing Schiff-base ligands. Unlike cisplatin — the gold-standard metal-based anticancer drug — ruthenium compounds can switch oxidation states inside the cell, potentially offering improved selectivity and lower toxicity.

For every compound I synthesise, I run a parallel computational study: DFT calculations reveal the electronic structure, HOMO–LUMO gaps predict reactivity, MEP maps show where the molecule wants to bind, and molecular docking places it inside a target protein to estimate affinity.

Lab results then confirm — or challenge — what the computer predicted. This dialogue between computation and experiment is the engine of my research.

View Publications →
⚗️

Synthesis & Characterisation

Multistep organometallic synthesis confirmed by ¹H/¹³C NMR, FT-IR, UV-Vis, single-crystal X-ray diffraction, and CHNS elemental analysis.

💻

Computational Analysis

DFT geometry optimisation, HOMO–LUMO, MEP surface mapping (GAUSSIAN), and molecular docking studies (AutoDock, Discovery Studio) with ADME profiling.

🔬

Biological Evaluation

Anticancer, antimycobacterial, antioxidant, and DNA-binding assays conducted with partner laboratories; results integrated into SAR analyses.

📊

Structure–Activity Relationships

Substituent effects, coordination geometry, and electronic parameters correlated with biological potency across 10+ complexes.

Specialisations

Core Research Areas

🧬

Ruthenium Metallodrugs

Designing Ru(III) and half-sandwich Ru(II)–arene complexes as next-generation anticancer and antimycobacterial agents with selectivity advantages over cisplatin.

Organometallic SynthesisNMR / X-ray DiffractionDNA Binding StudiesAnticancer Assays
💻

Computational Drug Design

Applying DFT, HOMO–LUMO analysis, MEP mapping, and molecular docking to rationalise reactivity and predict binding affinities.

GAUSSIAN (DFT)AutoDock / Discovery StudioHOMO–LUMO & MEPADME Prediction
⚗️

Structure–Activity Relationships

Correlating electronic structure, ligand substitution patterns, and molecular geometry with experimental biological activity to understand structure–activity relationships across a series of ruthenium complexes.

SAR AnalysisElectronic Descriptor AnalysisSubstituent Effect StudiesComputational–Experimental Correlation
🔬

Biological Evaluation

Collaborating on anticancer, antimycobacterial, antioxidant, and DNA-binding studies and integrating the experimental findings with computational analyses to support mechanistic interpretation.

MTT Anticancer AssayMIC AntimycobacterialDPPH AntioxidantDNA Binding Fluorescence
3D Structure

Piano-Stool Geometry

Half-sandwich [Ru(η⁶-p-cymene)(N,O-Schiff base)Cl]⁺ complexes adopt the distinctive piano-stool geometry: the η⁶-coordinated arene acts as the “seat”, while the bidentate Schiff base N,O donors and chloride ligand form the three “legs”.

This geometry is biologically significant — it exposes the chloride leaving group to aquation inside cells, while the arene ring controls lipophilicity and cellular uptake. Varying the Schiff-base substituents tunes HOMO–LUMO gaps, binding affinities, and ultimately anticancer potency.

Coordination number

6 (half-sandwich)

η⁶ arene

p-Cymene

Chelate ligand

N,O-Schiff base

Leaving group

Cl⁻ (aquation)

[Ru(η⁶-p-cymene)(N,O-Schiff base)Cl]⁺ · Piano-Stool Geometry

The η⁶ p-cymene arene ring (top) forms the “seat”; the Schiff-base N,O donors and chloride (bottom) form the “legs” — giving the characteristic piano-stool geometry. Based on compounds reported in ChemistrySelect (2025). Drag to rotate · scroll to zoom.

Ru (ruthenium)C (carbon)N (nitrogen)O (oxygen)Cl (chloride)η⁶ coordination
Computational Toolkit

Machine Learning & Python Libraries

Python-based open-source ecosystem for computational chemistry, cheminformatics, and machine learning — applied to drug discovery and metallodrug research.

⚗️

RDKit

Cheminformatics

Industry-standard Python library for cheminformatics. Used for molecule parsing (SMILES/SDF), fingerprint generation (Morgan, MACCS), substructure search, and property prediction in virtual screening pipelines.

SMILES parsingMorgan fingerprintsProperty filtersSAR analysis
🧠

DeepChem

ML / Drug Discovery

Deep learning library for drug discovery and quantum chemistry. Applied for graph neural networks on molecular graphs, activity prediction, and ADMET modelling of ruthenium complex candidates.

Graph conv modelsADMET predictionMultitask learningScaffold splits

Future Research Vision: AI-Driven Virtual Screening

A five-step AI pipeline that takes a large compound library and intelligently narrows it down to the most promising metal-complex candidates — ready for bench synthesis.

01

Library Preparation

Parse all candidate structures with RDKit; generate 3-D shapes; convert file formats for downstream analysis.

02

Drug-Likeness Filter

Apply Lipinski rules — checking molecular weight, water-solubility, and drug absorption potential — to keep only viable candidates.

03

AI Similarity Ranking

Use machine learning to score each molecule by how similar it is to known active Ru complexes from the literature.

04

Molecular Docking

Virtually place the top candidates inside the target protein using AutoDock and score how tightly each one binds (binding energy ΔG).

05

DFT Validation

Run quantum chemistry calculations on the shortlisted hits to verify their electronic structure and HOMO–LUMO gap. Best candidates go to the lab.

How AI Narrows Thousands to a Handful

🗂️

Compound Library

All candidate Ru–Schiff-base structures

10,000+
💊

Drug-Like Filter

Pass molecular weight & lipophilicity rules

~3,000
🤖

AI Similarity Ranking

Most similar to known active Ru complexes

Top 200
🎯

Molecular Docking

Strongest predicted protein binding (ΔG)

Top 20
⚛️

DFT Validation

HOMO–LUMO & electronic structure verified

5 leads
🧪

Ready for Synthesis

Shortlisted for the wet lab

Best 2–3

Each stage filters smarter — only the most promising candidates reach the bench

Future Research Vision

Multi-Agent AI for Computational Drug Design

During my PhD, designing a Ru complex, running DFT in GAUSSIAN, docking in AutoDock, and extracting SAR insights was done manually — one compound at a time. Here is how specialised AI agents could automate that exact same workflow.

👩‍🔬

Chemistry Researcher

Sets the goal: Ru scaffold · substituent library · target protein (e.g. PARP-1 / HSA)

🤖

Orchestrator

Receives the research goal, breaks it into tasks, and assigns each to a specialised agent in sequence

Agent 1
🧪

Design Agent

RDKit · DeepChem

Enumerates new Ru–Schiff-base candidates and scores predicted biological activity using ML models

Candidate library

Agent 2
⚛️

DFT Agent

GAUSSIAN

Runs B3LYP/LANL2DZ calculations automatically, extracts HOMO–LUMO gap and MEP surface data

Electronic descriptors

Agent 3
🎯

Docking Agent

AutoDock · Discovery Studio

Docks each candidate into the target protein binding pocket and returns predicted binding affinity (ΔG)

Binding affinity scores

Agent 4
📊

SAR Agent

Statistical Analysis

Correlates electronic structure with binding affinity across the full series and ranks the best leads

Ranked lead compounds

🧫

Shortlisted Lead Compounds

Top-ranked Ru complexes — filtered by HOMO–LUMO gap, binding ΔG, and ADME — passed directly to bench synthesis, saving weeks of manual iteration

10–100× Faster Iteration

Parallel DFT jobs and automated docking replace sequential manual steps, compressing weeks into hours.

🤖

Automated SAR Analysis

The SAR Agent correlates electronic descriptors with binding affinity across the full series — no spreadsheet required.

🎯

Intelligent Lead Prioritisation

Agents rank candidates by HOMO–LUMO gap, docking ΔG, and ADME filters before a single milligram is synthesised.

🔄

Closed-Loop Design

Experimental results from the bench feed back into the Design Agent, continuously refining the next generation of candidates.

From Idea to Discovery

Research Workflow

01

Molecular Design

Design Ru(III) and half-sandwich Ru(II)–arene Schiff-base complexes based on coordination chemistry principles and literature-guided ligand selection.

02

Synthesis

Multistep organometallic synthesis of Ru(III) and half-sandwich Ru(II)–arene Schiff-base complexes.

03

Characterisation

¹H/¹³C NMR · FT-IR · UV-Vis · Single-crystal X-ray (where applicable) · CHNS elemental analysis.

04

Computational Analysis

DFT geometry optimisation, HOMO–LUMO, MEP maps, and molecular docking in GAUSSIAN / AutoDock.

05

Biological Evaluation

Anticancer, antimycobacterial, antioxidant assays; DNA binding kinetics via fluorescence.

06

Structure–Activity Interpretation

SAR analysis correlates substituent effects with potency to guide the next synthetic cycle.